Regulation of glycolysis in the early development of fish embryos

  • 3.41 MB
  • English
S. Karger , Basel, London[etc]
StatementL.S. Milman and Yu.G. Yurowitzky.
SeriesMonographs in developmental biology -- Vol.6
ContributionsYuroitzky, Yu. G.
ID Numbers
Open LibraryOL20011593M

Regulation of glycolysis in the early development of fish embryos. Basel ; New York: S. Karger, (OCoLC) Document Type: Book: All Authors / Contributors: L S Milʹman; I︠U︡ G I︠U︡rovit︠s︡kiĭ. Regulation of glycolysis in the early development of fish embryos.

Monogr Dev Biol. ;6(0) Regulation of glycolysis in the early development of fish by: 1. Author(s): Milman,L S; Yurowitzky,Yu G Title(s): Regulation of glycolysis in the early development of fish embryos [by] L.

Milman and Yu. Yurowitzky. Early Development in Fish In recent years, the teleost fish Danio rerio, known as the zebrafish, has become a favorite organism of those who wish to study vertebrate development.

Zebrafish have large broods, breed all year, are easily maintained, have transparent embryos that develop outside the mother (an important feature for microscopy), and Cited by: 3. Fructose 2,6 P 2 and PFK-2 have a key role in the regulation of glycolysis-gluconeogenesis in fish 2.

PFK-1 and FBPase-1, as in mammals, are the target enzymes for fructose 2,6 P 2, this in turn may be controlled by glucagon and by:   PFKFB4 is an enzyme known to regulate glycolysis.

The authors reveal a surprising new role for this protein in global patterning of dorsal ectoderm in frogs that is Regulation of glycolysis in the early development of fish embryos book by Akt signalling and. Cleavage in most frog and salamander embryos is radially symmetrical and holoblastic, just like echinoderm cleavage.

The amphibian egg, however, contains much more yolk. This yolk, which is concentrated in the vegetal hemisphere, is an impediment to cleavage.

Description Regulation of glycolysis in the early development of fish embryos EPUB

Thus, the first division begins at the animal pole and slowly extends down into the vegetal region (Figure ; see. Non‐invasive microanalytical methods have been devised to study the energy metabolism of single human preimplantation embryos. Psyruvate, which is added routinely to all media used to culture human embryos, is consumed throughout the preimplantation period, with glucose assuming an increasing role at embryo compaction and blastocyst formation.

To better understand the different stages of fish embryo development, we should provide some background on basic embryology concepts. These include understanding different egg types and the phases which make up early embryonic development.

Different eggs are often differentiated by how much yolk is contained within and how it is distributed. The yolk is the part of the egg. The stages of embryonic development observed with cleavage, followed by blastula, morula, early gastrula, middle gastrula, late gastrula and until hatching of.

Book Series Menu × Monographs in Developmental Biology Genetic and Biochemical Aspects of the Development of Datura: TOC: Vol. 11, Histogenesis and Morphogenesis in Planarian Regeneration Vol. 7, Morphogenesis of T-Even Bacteriophages: TOC: Vol.

6, Regulation of Glycolysis in the Early Development of Fish Embryos: TOC. During its complete combustion, glucose is first processed in glycolysis, generating two molecules of pyruvate that are later fully oxidized in the Krebs cycle (Fig.

2A). However, instead of entering the Krebs cycle, glucose derivatives produced in glycolysis can be used in anabolic biosynthetic reactions (Fig. 2A) For instance. Glycolysis has previously been shown to be important for very early embryonic development, with its use decreasing significantly with age34 35 At these early.

Dear Colleagues, This Special Issue of the International Journal of Molecular Science shall encompass topics regarding the evolution of regulation of gene expression during eukaryotic embryonic development. Topics may include but not be limited to the evolution of differential gene expression at the level of transcription, mRNA processing, translation, and/or subcellular.

Moreover, the mRNA level of egln3a was significantly affected by the diet at first-feeding in fish exposed to the early mgL −1 O 2 hypoxic stimulus, either at the embryo.

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Previously, it has been revealed that antidepressant exposure significantly inhibits the growth and development of fish during their early developmental stages. Thus, in the present study, we aimed to identify and compare the underlying mechanisms of action of different antidepressants at the transcriptional level using zebrafish (Danio rerio.

Purchase The Zebrafish: Cellular and Developmental Biology, Part B, Volume - 3rd Edition. Print Book & E-Book. ISBN  Pesticides and insecticide toxicity can be assessed by the quantitative study of early development in aquatic organisms [13,14]. Fish embryos and larvae are extremely sensitive to environmental pollutants, which can result in morphological changes, and such biomarkers can be used to assess the influences of pollutants on aquatic animals.

This particular isoform of PK has been shown to promote aerobic glycolysis and anabolic metabolism (), and recently has been identified in the mammalian blastocyst (). Further work is warranted on establishing the regulation of PKM2 in the embryo, with specific reference to its control by exogenous factors and signaling pathways involved.

Stages are named, not numbered as in most other series, providing for flexibility and continued evolution of the staging series as we learn more about development in this species.

The stages, and their names, are based on morphological features, generally readily identified by examination of the live embryo with the dissecting stereomicroscope. Gametes, Embryos and Early Development Justin St. John* Centre for Reproduction and Development, Monash Institute of Medical Research, Clayton, VIC *Corresponding author: @ Fig.

Regulation of mtDNA copy number during development. Previous studies have identified roles for GDF6 orthologs during early embryonic and neural crest development, but have not identified direct regulation of melanocyte development by GDF6.

Here, we investigate the BMP ligand gdf6a, a zebrafish ortholog of human GDF6, during the development of melanocytes from the neural crest. Early blastocyst is the fifth day embryo development stage which has a blastocoel less than 50% of the embryo volume, and the formation of ICM and TE is not clear.

Figure 8. Blastocyst (grade 2: A: A) embryos that have embryo development stage with a grade of 2 are blastocyst embryos which still have thick ZP. Given the necessity of glucose consumption in the developing embryo, to investigate the impact of metabolic regulation on HSC formation, we upregulated nutrient input: wild-type (WT) zebrafish embryos were exposed to D-glucose (1% in fish water) from 5 somites to 36 hpf, throughout hematopoietic initiation.

After an egg is fertilized, and has fused with sperm to form a zygote, an embryo begins to develop. The process in which an organism develops from a single-celled zygote to a multi-cellular organism is complex and well-regulated.

The early stages of embryonic development are also crucial for ensuring the fitness of the organism. -Regulation of Cell Division. -The embryo of Zebra Fish-Vertebrate development. -Clear embryos-Can study early development w/o damaging cell.

What is studied in Tansgenic Mice. What characteristics allow this?-Closely related to humans-Study various human disorders/diseases. To further examine the concept of egg quality and the physiology of stored salmonid eggs, we investigated the effects of different oxygen tensions on the adenosine triphosphate (ATP) levels of unfertilized, activated, and fertilized chinook salmon (Oncorhynchus tshawytscha) eggs.

The ATP levels of unfertilized chinook salmon eggs were ± nmol ATP per egg. PGD involves the screening of human embryos as part of the process of in vitro fertilization, during which embryos are conceived and grown outside the mother’s body for some period of time before they are implanted.

The term PGD usually refers to both the diagnosis, selection, and the implantation of the selected embryos. The Egg Figure Frog Egg. The frog egg is a huge cell; its volume is over million times larger than a normal frog cell.

During embryonic development, the egg will be converted into a tadpole containing millions of cells but containing the same amount of organic matter. The resulting embryo is not genetically viable and dies within a few days.

Cleavage and Blastula Stage. The development of multi-cellular organisms begins from a single-celled zygote, which undergoes rapid cell division to form the blastula.

The rapid, multiple rounds of cell division are termed cleavage. Cleavage is illustrated in (Figure. The development of insulin-producing pancreatic beta (β)-cells represents the culmination of a complex developmental program. Cells of the posterior foregut assume a pancreatic identity, cells within the expanding pancreatic primordia adopt an endocrine fate, and a subset of these precursors becomes competent to generate β-cells.

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Postnatally, β-cells are primarily .Figure Frog embryo courtesy L. M. Beidler. A fertilized egg is much larger than the normal cells of an animal's body.

Some (e.g., a hen's egg) are truly huge. The frog egg has a volume millions times larger than a typical frog cell. The photo is of a cell frog embryo. This mass of cells is no larger than the original egg.Fish and embryos are exposed for 14 days and hours post fertilization, respectively, to – μg/L and – μg/L BP Chemical analysis of water and fish demonstrates more» that BP-3 is partly transformed to benzophenone-1 (BP-1) and both compounds are accumulated in adult fish.